摘要
目的：探讨乳腺癌基因（ＢＲＣＡ１）在遗传性卵巢癌发生、发展中的作用，并分析ＢＲＣＡ１ 基因杂合性丢失（ＬＯＨ）。方法：应用多聚合酶链反应技术（ＰＣＲＴ），对１０份遗传性卵巢癌组织中ＢＲＣＡ１ 基因内部的Ｄ１７Ｓ８５５ 微卫星位点进行ＬＯＨ检测。结果：２份发生杂合性丢失，ＬＯＨ发现率为２０％ 。结论：ＢＲＣＡ１在遗传性卵巢癌中具有肿瘤抑制基因的作用，同时不能排除其他肿瘤抑制基因的作用。
Objective:To evaluate the role of BRCA1 gene in the development and progress of hereditary ovarian cancer,and to investigate the loss of heterozygosity(LOH)at the BRCA1 genetic loci,D17S855.Methods:Using the polymerase chain reaction technique(PCRT),LOHs in 10 samples of hereditary ovarian cancer at intragenic loci were detected.Results:Only 2/10 samples(20%) had LOH.Conclusions:BRCA1 is a tumor surpressor gene in hereditary ovarian cancer,but other tumor supressor genes may also exist.
出处
《现代妇产科进展》
CSCD
1999年第3期209-212,共4页
Progress in Obstetrics and Gynecology
关键词
卵巢癌
遗传性
BRCA1
基因杂合性丢失
Ovarian cancer tissues,hereditary
Gene,BRCA1
Loss of heterozygosity(LOH)
