摘要
目的对不同产地、不同表型性状的砂仁Amomi Fructus种质资源进行遗传多样性分析。方法采用简单序列重复区间(ISSR)扩增技术,对来自云南、海南、广东、福建等地区的21个砂仁样品进行遗传多样性及亲缘关系分析;同时,对其中16个阳春砂仁的株高、茎粗、叶片数、叶片大小等部分植物学表型性状进行测定,利用这些数据对各居群砂仁进行聚类分析。结果 ISSR分析结果显示,从60条ISSR引物中筛选出11条用于扩增,共检测出54条DNA条带,多态性位点为22个,多态性条带比率(PPB)可达40.74%,Nei’s基因多样性(H)为0.116 1,Shannon’s多态性信息指数(I)为0.184 2;各居群表型性状差异较小。结论砂仁种质资源的遗传多样性较低。
Objective To evaluate the genetic diversity ofgermplasm resources for Amomi Fructus from various habitats in different phenotypes. Methods Inter-simple sequence repeats （ISSR） markers were used to analyze the genetic diversity and the genetic relationship among 21 samples ofAmomi Fructus collected from Yunnan, Hainan, Guangdong, and Fujian Provinces; At the same time, the phenotypic characters of 16 Amomum villosum samples were measured in height, stem diameter, blade number, and blade size, etc. Then cluster analysis on all populations of Amomi Fructus was carried out based on the above data. Results Eleven primers selected from 60 ISSR-primers were used for amplification and a total of 54 DNA bands were obtained,including 22 polymorphic bands. At species level, the ayerage percentage ofpolymorphic bands （PPB） was 40.74%, Nei＇s geme diversity （H） was 0.116 1, Shannon＇s information index （/）was 0.184 2. There was a little difference of morphological characteristics. Conclusion The genetic diversity of germplasm resources in Amomi Fructus is lower.
出处
《中草药》
CAS
CSCD
北大核心
2011年第3期570-574,共5页
Chinese Traditional and Herbal Drugs
基金
云南省重大创新工程(2008IF018)
关键词
砂仁
ISSR
遗传多样性
表型性状
聚类分析
Amomi Fructus
inter-simple seauence repeats （ISSR）
genetic diversity
phenotypic characteristic
cluster analysis
