摘要
【目的】探讨十溴联苯醚对小鼠体外受精及受精卵早期发育的毒性作用,并探究其剂量效应关系。【方法】按照体外受精培养液中BDE-209终浓度的不同将实验分为4组:A组,0μg/mL;B组,10μg/mL;C组,20μg/mL;D组,40μg/mL。取性成熟昆明雌鼠20只及雄鼠8只,对雌鼠行促排卵及促卵成熟,收集成熟卵母细胞随机平均放入以上培养液中,对雄性小鼠附睾尾取精,加入培养液中,使其受精。放入37℃,体积分数为5%CO2培养箱中培养。统计2-细胞成形率、4-细胞成形率、8-细胞成形率、桑葚胚成形率及囊胚成形率。【结果】在2-细胞期,B组受精率为63.3%,与对照组的69.2%相比,差异无统计学意义(P>0.05),但C组(28.1%)和D组(24.1%)明显低于对照组(P<0.01)。2-细胞、4-细胞、8-细胞、桑葚胚及囊胚形成率,C组均明显低于B组(P<0.01);D组除2-细胞期外,均显著低于C组(P<0.01)。【结论】BDE-209可导致小鼠体外受精率降低,影响受精卵的早期发育,并且呈现明显的剂量效应关系。
【Objective】 To investigate the toxic effects on the mice in vitro fertilization and early development of fertilized eggs induced by polybrominated diphenyl ethers（BDE-209）,and explore the dose-response relationship.【Methed】The culture medium was divided into four groups according to the final concentration of BDE-209： Control group A： 0 g/mL;Group B： 10 g/mL;Group C： 20 g/mL;Group D： 40 g/mL.Sexually female and male mature Kunming mice were taken respectively 20 and 8.The female mice and mature eggs were ovulated with hormone.Mature oocytes were collected and placed in the culture medium randomLy.Then the sperm was gained from epididymal tail of the mice,and put into the medium.The disk was put into the incubator（37℃,Volumefanction of CO2 is 5%） to fertilizate and culture the cells.Finally,the forming rates of 2-cells,4-cells,8-cells,morula and blastocyst were calculated.【Result】 There was no difference between group A（69.2%） and group B（63.3%） in the 2-cells stage（P 0.05）,but the forming rates of group C（28.1%） and group D（24.1%） were significantly lower than that of group A（P 0.01）.The forming rates of 4-cells,8-cells,morula and blastocyst,all the experimental groups were significantly lower than that of the control group（P 0.01）.【Conclusion】 BDE-209 could decrease the forming rates of the mice in vitro fertilization and lead toxic effects on the early development of fertilized eggs.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2011年第1期51-55,共5页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省医学科学研究基金(A2010325)
关键词
十溴联苯醚
体外受精
受精卵
毒性作用
polybrominated diphenyl ethers
in vitro fertilization
zygote
toxic effects
