摘要
【目的】建立SD大鼠自体原位肝移植模型,观察自体模拟肝移植后大鼠肾功能与病理学变化特点。【方法】SD大鼠40只,随机分为对照组(C组,n=8)和模型组(M组),模型组根据肝脏再灌注时间,再分为再灌注4 h模型组(M1组,n=8),再灌注8 h模型组(M2组,n=8),再灌注16 h模型组(M3组,n=8),再灌注24 h模型组(M4组,n=8)4个亚组。对照组在麻醉后只进行开腹和血管的分离,不进行肝脏的阻断和灌注;模型组则进行自体肝移植手术,分别在肝脏再灌注后4、8、16、24 h时取左肾组织和血标本,观察其血肌酐、尿素氮和病理形态学变化。【结果】与C组相比,模型组大鼠肾脏在肝脏再灌注后出现明显的病理学损伤,可见间质出现较多炎性细胞浸润,肾小管扩张,部分肾小管上皮细胞变性、坏死、脱落,较多管型形成,在再灌注8 h时最为显著,在再灌注16 h开始修复;血清肌酐和尿素氮在肝脏再灌注后明显升高,在再灌注后16 h达到高峰,在24 h恢复。【结论】SD大鼠经历自体原位肝移植后,肾脏出现病理损伤和功能损害。
【Objective】To investigate function and pathological change in kidney after orthotopic liver autotransplantation in the SD rat model.【Methods】 Forty Sprague-Dawley rats were randomly divided into control group（Group C,n = 8） and model group（Group M）.According to the time of liver reperfusion,the rats in Group M were divided into 4 h after reperfusion（Group M1,n = 8）,8 h after reperfusion（Group M2,n = 8）,16 h after reperfusion（Group M3,n = 8）,24 h after reperfusion（Group M4,n = 8）.Group C received sham operation after anesthesia,which involved laparotomy and vascular dissection.Rats in other groups received orthotopic liver autotransplantation.Left kidneys were removed and blood sample was collected at 4 h,8 h,16 h,and 24 h after reperfusion depending on different groups.Concentrations of Serum creatinine（Scr）,blood urea nitrogen（BUN） and pathological changes were detected.【Results】 In model groups,morphological damage in kidney was significant when comparing to group C especially at 8 hour after reperfusion.Kidneys showed inflammatory cell infiltration in renal interstitium,tubular dilatation,degeneration of tubular epithelial cell,necrosis,tubular lumen obstruction after reperfusion.The most significant pathological damage was detected at 8h after reperfusion.Kidneys were showed repair of pathological damage at 16 h after reperfusion.Furthermore,elevated concentrations of Cr and BUN in model groups were observed and peaked at 16 h after reperfusion,then concentrations of Scr and BUN were dropped to baseline at 24 h after reperfusion.【Conclusion】 Funcion and pathological change in kidney were significant after orthotopic liver autotransplantation in the SD rat model.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2011年第1期47-50,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
国家自然科学基金(30972858
30801080)
广东省科技计划项目(2008B060600020
2009B050700021
2010B031600040)
关键词
自体原位肝移植
急性肾损伤
病理学
orthotopic liver autotransplantation
acute kidney injury
pathology
