摘要
目的:观察牡蛎提取物对酒精性肝损伤大鼠IL-17、TNF-α的影响及其对肝脏的保护作用.方法:采用给予酒精灌胃制造酒精性肝损伤模型的方法,将75只♂Wistar大鼠随机均分为空白组、模型组以及3个实验组即牡蛎提取物低剂量、中剂量、高剂量共5组.模型组仅给予500mL/L的酒精灌胃8wk,实验组给予同等量酒精灌胃的同时还分别给予低剂量、中剂量和高剂量牡蛎提取物8wk.空白组每天仅给予等量蒸馏水灌胃8wk.实验结束后,抽取大鼠腹主动脉血液,检测各组大鼠血清IL-17、TNF-α、ALT与AST的含量并分析.结果:空白组及牡蛎提取物低、中、高剂量组血清IL-17、TNF-α、ALT和AST显著低于模型组(IL-17:2.90ng/L±0.56ng/L,14.34ng/L±0.64ng/L,11.31ng/L±0.58ng/L,8.33ng/L±0.64ng/Lvs15.68ng/L±0.97ng/L;TNF-α:23.94ng/L±1.07ng/L,40.93ng/L±0.98ng/L,36.46ng/L±0.99ng/L,31.44ng/L±1.57ng/Lvs43.62ng/L±1.02ng/L;ALT:53.15U/L±3.57U/L,64.56U/L±7.39U/L,60.64U/L±8.46U/L,58.53U/L±5.63U/Lvs79.46U/L±5.65U/L;AST:110.25U/L±6.65U/L,134.15U/L±6.52U/L,121.57U/L±6.75U/L,118.28U/L±4.49U/Lvs150.46U/L±10.47U/L,均P<0.05).结论:牡蛎提取物可以有效地降低由酒精引起的IL-17、TNF-α及转氨酶的升高,有明显的保肝降酶的作用.
AIM： To investigate the effect of oyster extract on serum levels of interleukin-17 （IL-17） and tumor necrosis factor-α （TNF-α） in rats with alcoholic liver injury. METHODS： Seventy-five male Wistar rats were randomly divided into five groups： normal control group, model group, low-, medium- and high-dose oyster extract groups. Except the normal control group, rats of the other groups were subjected to induction of alcoholic liver disease by intragastric infusion of alcohol （500 mL/L） once a day for 8 wk. The three oyster extract groups were additionally given different doses of oyster extract once a day for 8 wk. The normal control group was given equal volume of distilled water for the same duration. After treatment, all rats were killed to measure serum levels of IL-17, TNF-α, alanine aminotransferase （ALT）, and aspartate aminotransferase （AST）. RESULTS： The levels of serum IL-17, TNF-α, ALT, and AST in the normal control group and low, medium- and high-dose oyster extract groups were significantly lower than those in the model group （IL-17：2.90 ng/L ± 0.56 ng/L, 14.34 ng/L ± 0.64 ng/L, 11.31 ng/L ± 0.58 ng/L, 8.33 ng/L ± 0.64 ng/L vs 15.68 ng/L±0.97 ng/L; TNF-α： 23.94 ng/L ± 1.07 ng/L, 40.93 ng/L ± 0.98 ng/L, 36.46 ng/L ± 0.99 ng/L, 31.44 ng/L ± 1.57 ng/L vs 43.62 ng/L ± 1.02 ng/L; ALT： 53.15 U/L ± 3.57 U/L, 64.56 U/L ± 7.39 U/L, 60.64 U/L ± 8.46U/L, 58.53 U/L ± 5.63 U/L vs 79.46 U/L ± 5.65 U/L; AST： 110.25 U/L ± 6.65U/L, 134.15 U/L ± 6.52U/L, 121.57 U/L ± 6.75U/L, 118.28 U/L ± 4.49U/L vs 150.46 U/L ± 10.47 U/L; all P 〈 0.05）. CONCLUSION： Oyster extract attenuates alco- hol-mediated increase in serum levels of IL-17, TNF-α, ALT and AST in rats with alcoholic liver injury.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第2期177-180,共4页
World Chinese Journal of Digestology
