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PLK1与P53在食管鳞癌细胞中的表达及相关性研究 被引量：2

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摘要 目的:探讨质粒表达载体介导的RNA干扰抑制PLK1基因表达对人食管鳞癌细胞Eca-109的P53基因的影响。方法:构建靶向人PLK1基因的RNA干扰表达质粒(PLK1-shRNA),将该干扰质粒转染到人食管鳞癌细胞Eca-109中,采用Westernblot技术分析检测RNA干扰后48h、72h各组细胞PLK1基因和P53基因蛋白表达水平的变化。结果:Westernblot结果显示,转染RNA干扰表达质粒(PLK1-shRNA)后,PLK1-shRNA组Eca-109细胞48h、72h较转染液对照组的PLK1基因蛋白表达分别降低了83.7%和56.0%(P<0.05);PLK1-shRNA组Eca-109细胞48h、72h较转染液对照组的P53基因蛋白表达分别升高了46.0%和70.5%(P<0.05)。结论:RNA干扰PLK1基因能够有效地抑制Eca-109细胞中PLK1基因的表达,从而有效地升高P53基因的表达,说明PLK1基因抑制P53基因在Eca-109细胞中的表达。
作者 殷力佳 陈德玉 胡琴
机构地区 江苏大学附属医院放疗科
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2011年第2期224-226,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 国家自然科学基金资助项目(30872335)
关键词 PLK1 P53 RNA干扰 ECA-109细胞
分类号 R392.33 [医药卫生—免疫学]
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参考文献6

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共引文献7

同被引文献36

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细胞与分子免疫学杂志
2011年 第2期

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