摘要
核小体是真核细胞染色质的基本单位。真核基因组ＤＮＡ在细胞核中储存在染色质结构中。核小体的核心结构是由１４６个碱基对的ＤＮＡ盘绕四对核心组蛋白的八聚体外面近两周所构成。核小体核心组蛋白Ｈ３／Ｈ４两对异源二聚体是核小体亚颗粒的核心，它能稳定地与ＤＮＡ结合...
Abstract The suppression of eukaryotic RNA synthesis by in vivo core histones has been known for more than 30 years. Detailed mechanisms for its regulation has not been accessible until the middle of the 90s.Ordered nucleosome arrays can first be reconstituted by individually cloned Drosophila recombinant or biochemically purified components.Chromatin remodeling can now be mimmiced in vitro with dynamiclly reconstitute nucleosomes.When DNA binding proteins are available and bind to their specific elements in the presence of chromatin remodeling or modification factors,chaperones,and ATP,chromatin structure transits from its original inactive state through derepressed state to an open conformation and leads to promote gene transcription.The nature of chromatin remodeling,the role of histone acetyl transferase and the deacetylase,the inhibition mechanism of DNA methylation through the methylated CpG binding proteins,as well as the importance and necessities of chromatin study in the era of functional human genomics have been discussed.
出处
《生物工程进展》
CSCD
1999年第4期23-27,10,共6页
Progress in Biotechnology
