摘要
[目的]探讨端粒酶RNA（TERC）基因在宫颈癌和癌前病变中的表达及临床意义.[方法]采用双色荧光原位杂交技术（FISH）以TERC/CSP3 DNA探针检测26例宫颈癌及69例宫颈上皮内瘤变（CIN）脱落细胞中TERC基因的表达.[结果]CINⅠ、CINⅡ、CIN Ⅲ和宫颈癌TERC基因阳性表达率分别为29.2%（7/24）、57.7%（15/26）、89.5%（17/19）、100%（26/26）,炎症组、CINⅠ、C INⅡ、C IN Ⅲ和宫颈癌组的TERC基因阳性扩增量分别为0.46±1.33,0.58±1.32,1.62±1.90,13.32±21.23,51.0±31.69.TERC基因阳性扩增率和扩增量随着病变程度的加重而增高（P〈0.01）.[结论]FISH技术能定量检测TERC基因并有助于鉴别细胞的恶性程度,可作为宫颈癌的诊断手段之一.
[Objective] To explore the expression of telomerase RNA（TERC） gene in cervical carcinoma and cervical intraepithelial neoplasia（CIN） and its clinical significance. [Methods] Two color fluorescence in situ hybridiza tion（FISH） with TERC/CSP3 probe was used to detect the expression of TERC gene in the ceils of 26 patients with cervical carcinoma and 69 patients with CIN. [Results] The positive rate of TERC gene inCINⅠ、C INⅡ、C IN Ⅲ and cervical carcinoma was 29.2% （7/24）, 57.7 % （ 15/26）, 89.5% （17/19） and 100 % （26/26）, respectively. The amplification level of TERC gene in the inflammation, CINⅠ、C INⅡ、C IN Ⅲ and cervical carcinoma was0.46±1.33,0.58±1.32,1.62±1.90,13.32±21.23,51.0±31.69. respectively. As the cervical lesions worsened, the positive rates and degree of TERC gene amplification obviously increased（ P〈0.01）. [Conclusion] FISH technique can be used as the quantitive detection of TERC gene, and is helpful for the identification of the malignant levels of cells. Therefore it is one of the diagnostic methods of cervical cancer.
出处
《医学临床研究》
CAS
2011年第2期271-272,共2页
Journal of Clinical Research
