摘要
为实现快速准确定量水体的藻细胞浓度,提出一种藻细胞浓度的虚拟采样检测方法。该方法首先采用聚苯乙烯标准颗粒溶液标定配置有CCD(Charge Coupled Device)相机的倒置显微镜的虚拟采样容积,然后使用该倒置显微镜对藻细胞溶液进行成像,最后采用数字图像处理技术处理图像并得到藻细胞浓度结果。结果表明,同一藻细胞溶液进行连续2倍率稀释后获取6个样品,对样品的检测结果做一阶线性回归分析,一次项系数为1.030,相关系数为0.999 2。这表明虚拟采样容积在不同浓度的藻细胞溶液中数值稳定,藻细胞浓度的虚拟采样检测方法正确。
This paper presents a method for measuring algae cell concentrations via virtual sampling.The method is suitable for detecting the biomass of algae in the water,with the standard solution used to calibrate the virtual sample volume of an inverted microscope equipped with charge coupled device（CCD） digital camera,with the inverted microscope used to capture images of the algae solution,and with the digital image processing then used to analyze the picture and determine the concentration of algae cells.Experiments with a linear regression program applied to 6 samples diluted continuously from one algae solution show that the first-order coefficient is 1.030 and the correlation factor is 0.999 2,which indicates that the virtual sample volume value is stable in algae solutions of different concentrations and the method for algae cell concentrations via virtual sampling is accurate.
出处
《清华大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2010年第11期1807-1810,共4页
Journal of Tsinghua University(Science and Technology)
关键词
检测
藻
细胞浓度
虚拟采样容积
数字图像分析
measurement
algae
cell concentration
virtual sample volume
digital image analysis
