摘要
采用枸杞花药进行离体培养,建立细胞系,诱导植株再生,结果在含不同激素的4种培养基上都诱导出了愈伤组织,诱导率为17%～169%。愈伤组织在MS+2,4D05mg/L的固体培养基上,经2～3次培养后,获颗粒状胚性愈伤组织,颗粒状胚性愈伤组织转入含相同成分的液体培养基中进行振荡培养,24h后获得大量单细胞。单细胞液经过多次继代培养,建立起稳定的悬浮系。悬浮细胞在液体培养基中培养8～10d可获得含有大量胚状体的愈伤组织块,收集悬浮培养物转移到MS+6BA02mg/L的固体培养基上,胚状体能够萌发形成大量绿色小芽,小芽转入生根培养基(MS+NAA02mg/L)中20d后得到完整植株。植株根尖细胞经细胞学鉴定为单倍体。
Calli of anther of Lycium barbarum were induced on 4 MS media with different hormone contents. The induction rate is 1.7%～16.9%. The calli became small particls with good dispersion ability after 2～3 successive transfers of culture on the medium MS+2,4-D 0.5mg/L. When these particles were cultured on liquid medium, a lot of single cells were obtained after 24 hours. After several times of successive transfers of culture, a stable cell suspending culture line was established. After 8～10 days, a lot of embryoids appeared on the calli on the liquid medium. The embryonic calli were then moved onto solid medium (MS+6BA 0.2mg/L) and green buds appeared. After 20 days of culturing the buds on rooting medium, complete plantlets were obtained. The chromosome numbers from root tip somatic cells of regeneration plant are twelve. It shows that the regeneration plant is haploid.
出处
《云南植物研究》
CAS
CSCD
1999年第3期346-350,共5页
Acta Botanica Yunnanica
基金
国家科委九五攻关项目
关键词
枸杞
花药
悬浮培养
再生植株
Lycium barbarum, Anther, Suspending culture, Regeneration plant
