摘要
目的：建立内标法测定人血浆中去甲万古霉素质量的HPLC检测方法。方法：采用20%（200mg/mL）硫酸锌溶液直接沉淀血浆蛋白,以万古霉素为内标,HPLC法测定人血浆中去甲万古霉素的质量。结果：人血浆中杂质不干扰样品峰和内标峰,在低浓度（2.00μg/mL）、中浓度（20.00μg/mL）、高浓度（200.00μg/mL）的回收率分别为90.50%,93.70%和96.40%;批内精密度3种（低、中、高）浓度分别为8.50%,6.40%和5.30%,批间精密度分别为9.40%,7.50%和6.70%,最低检测限为0.50μg/mL,线性范围为1.00-300.00μg/mL。结论：本法稳定、便捷、精密度好,准确可靠,适用于去甲万古霉素血药浓度测定。
Objective： To establish the determination of norvancomycin in human blood plasma by HPLC.Methods： Plasma protein was precipitated with zinc sulfate Solution（20.00% or 200 mg/mL）,and vancomycin was used as internal standard.Plasma concentrations of norvancomycin and internal standard were determined by HPLC-PAD.Results： The chromatographic peak of norvancomycin and internal standard was not interfered by foreign matter.Recovery rate of 2.00,20.00,200.00 μg/mL was 90.50%,93.70%,96.40%,respectively.Accuracy in or between batches was less than 9.0% and l0.00%,respectively.The lowest detectable limit was 0.50 μg/mL and the linear range was 1.00~300.00 μg/mL.The freeze thawing of plasma was stable.Conclusion： The method was reliable and stable and it was suitable for determination of the concentration of norvancomycin in plasma.
出处
《抗感染药学》
2011年第1期36-38,共3页
Anti-infection Pharmacy
基金
盐城市科技发展项目(编号:yz2007015)
