摘要
目的探讨睡眠剥夺(sleep deprivation,SD)对大鼠动脉粥样硬化(atherosclerosis,AS)进展的影响,是否促进炎症发展及AS斑块的不稳定、破裂甚至诱发急性心脑血管事件发生及其机制。方法将60只Wistar雄性大鼠随机分为6组(每组10只):空白对照组(C组)、AS对照组(AS组)、AS SD 1d组(SD1d组)、AS SD3d组(SD3d组)、AS SD 5d组(SD 5d组)、AS SD 7d组(SD 7d组)。5组大鼠建立AS模型后,其中4组再建立SD模型,制备主动脉组织标本,HE染色,光镜观察切片。检测血清TNF-α、高敏C反应蛋白、白细胞介素6水平。检测主动脉组织内基质金属蛋白酶9水平。结果随着SD时间的延长,大鼠AS病变进展逐渐加重。血清中TNF-α、高敏C反应蛋白、白细胞介素6水平、主动脉组织内基质金属蛋白酶9水平逐渐增高。结论 SD可加速大鼠机体的炎症发展,引起不稳定斑块的出现从而促进AS进程。
Objective To study the effects of sleep deprivation（SD） on atherosclerosis（AS） pro gression in rats,and to make clear whether sleep deprivation can promote the development of inflammation,instability and rupture of atheroselerotie plaque and even induce the acute cardiovascular and eerebrovascular events and their mechanism in rats. Methods Sixty Wistar male rats were randomly divided into 6 groups（10 each） ： control group, AS group, SD 1 d group, SD 3 d group,SD 5d group,SD 7 d group. After AS model in 5 groups was established,SD model in 4 groups was established, the aortic tissue sections were prepared, stained with HE stain and ob- served by light microscopy. Serum TNF-a, hs-CRP, IL-6 levels and aortic tissue matrix metalloproteinase-9（MMP-9） levels were examined. Results With prolongation of SD in rats,AS lesions gradually worsened, MMP-9 level in aorta gradually increased, serum TNF-a, hs-CRP and IL 6 levels also increased gradually. Conclusion SD can accelerate the development of inflammation and induce appearance of instable plaques,thus contributing to the process of AS.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2011年第3期263-266,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
