摘要
为明确纳米金粒子对肿瘤组织近红外光学成像的影响,研究观察了肿瘤组织于纳米金粒子贮积后对其近红外光谱范围的光学特性的变化特点。实验中,应用MCG-803人胃腺癌细胞系（来源于CTCC）,采用原位接种法建立BALB/C裸小鼠胃癌模型,给予动物静脉注射纳米金粒子悬液,以组织切片形式检测肿瘤组织近红外光衰减系数的特征性变化。结果显示,纳米金粒子在肿瘤组织内贮积后,肿瘤组织在800-1500 nm的近红外光谱谱线特征未改变,基本轮廓相似,但其近红外光光谱透射率明显降低4.4%-7.0%,经900,1000,1100,1200,1300和1400 nm位点的数据统计,结果具有显著性差异。实验结果说明,纳米金粒子在胃癌裸鼠肿瘤组织中贮积后,可以使肿瘤组织近红光的衰减加强,为肿瘤组织的近红外光学成像检测提供参考。
In order to detect the effect of gold nanoparticles on gastric tumor tissue in near-infrared optical imaging,the spectral feature in near-infrared is observed after gold nanoparticles piled up in tumor tissues of gastric as a contrast agent.The MCG-803 human gastric cancer cell line（from CTCC） is subcutaneously inoculated in BALB/C nude mice,and then the orthotopic graft model of gastric cancer is made.Animals are divided into two groups： gastric tumor tissue control group and gastric tumor tissue with gold nanoparticles group.The optical attenuation coefficients of tumor tissue are detected by a near-infrared spectroscopy.The results show that the shape of the transmittance spectra of tumor tissues with gold nanoparticles and tumor tissues without gold nanoparticles is similar in near-infrared range from 800 to 1500 nm.But the spectral transmittance is slightly lower than that of the corresponding tumor tissue about 4.4% to 7.0%.And there are significant differences at the statistical sites of 900,1000,1100,1200,1300 and 1400 nm.The present study has suggested that gold nanoparticles accumulated in nude mice gastric cancer can affect on optical properties tumor of tissue,which can provide a theoretical guidance for the optical imaging detection of tumor tissue.
出处
《光学学报》
EI
CAS
CSCD
北大核心
2011年第3期201-205,共5页
Acta Optica Sinica
基金
国家自然科学基金(30873238)
广东省科技计划项目(2007B031401010)资助课题
关键词
医用光学与生物技术
纳米金
近红外光谱
光衰减系数
胃癌组织
medical optics and biotechnology
gold nanoparticles
near-infrared spectrum
optical attenuation coefficients
gastric tumor tissue
