摘要
对鹅血中SOD的纯化及抗氧化活性进行了研究。采用丙酮沉淀法对鹅血SOD进行初步纯化、Sepha-dex G-100进一步精制,并利用SDS-PAGE对酶的纯度检测和分子量测定。结果表明Sephadex G-100凝胶过滤层析对鹅血SOD具有较好的纯化作用,纯化后的鹅血SOD比活力从1 144.896U/mg提高到4 226.513U/mg,SDS-PAGE凝胶电泳显示单一条带,表明已达到电泳纯,且其亚基分子量约为16KD。纯化后的SOD对邻苯三酚自氧化抗氧化活性明显。
The preliminary purification of SOD from goose blood was carried out using acetone precipitation,followed by Sephadex G-100 further purification and SDS PAGE was used to detect the enzyme purity and molecular weight.The results showed that Sephadex G-100 played a good purification role for SOD from goose blood.The enzyme activity of SOD purification increased from 1144.896U/mg to 4226.513U/mg.SDS PAGE gel electrophoresis showed a single band,indicating the isolated SOD reached the electrophoretic purity,and its subunit molecular weight was about 16KD.And the antioxidant activity of the purified SOD to pyrogallic acid performed obviously.
出处
《肉类工业》
2011年第3期22-25,共4页
Meat Industry
基金
国家水禽产业技术体系资助(nycytx-45-14)
关键词
鹅血
纯化
抗氧化
goose blood
purification
antioxidant activity
