摘要
目的观察滋阴活血解毒饮片及超微方对大鼠局灶性脑缺血后白细胞介素-1β(IL-1β)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)含量的影响。方法将25只大鼠随机分为假手术组、模型组、滋阴活血解毒方饮片组(以下简称饮片组)、滋阴活血解毒方超微组(以下简称超微组)、阿加曲班组,每组5只。大脑中动脉线栓法复制局灶性脑缺血模型,进行神经功能评分。造模成功后7 d,用TTC染色法测定脑梗死范围,用Motic Med6.0数码图像分析系统分析脑梗死面积。采用ELISA法测定大鼠血清IL-1β、IL-8、TNF-α的含量。结果饮片组、超微组及阿加曲班组大鼠脑梗死体积比均明显低于模型组,差异具有统计学意义(P<0.01);模型组血清IL-1β、IL-8、TNF-α含量明显高于假手术组(P<0.01),饮片组、超微组、阿加曲班组均能明显降低大鼠血清IL-1β、IL-8、TNF-α含量,与模型组比较,差异均有统计学意义(P<0.01)。结论滋阴活血解毒方能减小脑梗死面积,抑制炎性因子的表达,降低脑缺血所致的脑损伤。
Objective To observe the different effects of traditional Ziyin Huoxue Jiedu decoction and its ultra-micro pieces decoction on the serum contents of IL-1β,IL-8 and TNF-α in rats.Methods 25 rats were randomly divided into the sham-operated,model,traditional Ziyin Huoxue Jiedu decoction（TZHJD）,ultra-micro pieces Ziyin Huoxue Jiedu decoction（UZHJD） and Argatroban Injection（AI） groups,5 rats per group.The models were reproduced by occluded middle cerebral artery.The neurological function were evaluated.After modeling 7 days,the cerebral infarct range was determined by TTC,the cerebral infarct area was analyzied by Motic Med6.0 digital image analysis system,and the serum content of IL-1β,IL-8 and TNF-α were measured by enzyme-linked immunosorbent assay（ELISA）methods.Results Compared with model group,infarct volum ratio in all the TZHJC,UZHJD and AI groups were lower（P0.01）,the content of IL-1β,L-8 and TNF-α in the model group was much higher than that in the sham-operated group（P0.01）,while the contents of IL-1β,IL-8 and TNF-α in all the TZHJC,UZHJD and AI groups were lower than that in the model group（P0.01）.Conclusion Zinyin Huoxue Jiedu decoction is effective to reduce cerebral infarct size,restrain inflammatory factor expression,and reduce the cerebral injury caused by cerebral ischemia.
出处
《湖南中医药大学学报》
CAS
2011年第1期36-39,共4页
Journal of Hunan University of Chinese Medicine
基金
湖南省科技厅资助项目(2009FJ3140)
湖南省教育厅青年基金项目(08B005)
