摘要
本文采用体外细胞培养法、细胞集落形成法、ＭＴＴ 比色法及３Ｈ－ ＴｄＲ掺入法探讨了西洋参多糖Ⅰ、Ⅱ、Ⅲ对肝癌细胞的增殖抑制作用。其结果表明癌细胞的ＤＮＡ 合成抑制率分别为５２－９ ％ 、１３－９％ 、１８－８ ％ ，细胞的存活率下降到４７－９ ％ 、７１－３２ ％ 、６８－３ ％ ，肿瘤干细胞的抑制率为６４－４ ％ ，２０－１ ％ ，２３－３ ％ 。且可见大部分细胞脱壁死亡或胞质粗糙，内有大量颗粒状物堆积。提示，西洋参多糖具有较强的抗癌作用。
The inhibitory effects of Panax polysaccharide Ⅰ、Ⅱ、Ⅲ(PPⅠ、PPⅡ、PPⅢ)on cell proliferation of hepatic carcinoma cell have been studied by cell culture、colony forming assay、MTT colorimetric assay、and 3H-TdR incorporation assay.The results show that the inhibitory rate of DNA synthesis of carcinoma cell is 52 9%、13 9%、18 8%individually,the survival rate descends to 47 9%、71 32%、68 3%,and the inhibitory rate of tumor stem cell is 64 4%、20 1%、23 3%.It is also inspected that most cells die of losing cell wall or cell plasma is rough in which there are many granular sediments.It is suggested that Panax polysaccharide has a good anti carcinoma effect on carcinoma cell.
出处
《临床肝胆病杂志》
CAS
北大核心
1999年第4期213-214,共2页
Journal of Clinical Hepatology
