摘要
研究不同剂量60Co γ射线辐照(10～50Gy)对地被菊种子发芽及相关生理指标的影响。结果发现:30Gy以上辐照剂量显著降低种子发芽率和幼苗成活率;随着辐照剂量的增加,20Gy以上剂量辐照造成了幼苗丙二醛(MDA)含量和过氧化物酶(POD)活性的显著升高,超氧化物歧化酶(SOD)和谷胱甘肽还原酶(GR)活性也呈升高趋势,且在40Gy以上辐照剂量下差异显著,过氧化氢酶(CAT)活性则表现为先升高后降低;除了40Gy处理有显著差异外,辐照基本没有影响抗坏血酸过氧化物酶(APX)活性。由此表明,地被菊种子的适宜诱变剂量为20Gy,辐照造成了成活幼苗膜脂过氧化损伤,但植株可通过提高抗氧化酶CAT和POD的活性来启动相应保护机制以减少伤害。
The seeds of ground-cover chrysanthemum were used to study the effects of different doses of 60↑Coγrays irradiation（10 ～ 50Gy） on seed germination and physiological characteristics.The results showed that the rate of seed germination and seedling survival decreased significantly with the irradiation doses.With the increase of irradiation dose to above 20Gy,the content of malondialdehyde（MDA） and activity of peroxidase（POD） in seedlings significantly increased.The similar trends were found in the activities of superoxide dismutase（SOD） and glutathione reductase（GR）.Catalase（CAT） activity increased at doses lower than 20Gy,and then decreased at the higher doses,whereas ascorbate peroxidase（APX） activity did not alter except for 40Gy.It is concluded that the suitable irradiation dose of mutation breeding is 20Gy for the seeds of ground-cover chrysanthemum.Although 60↑Coγrays irradiation resulted in damage of membrane lipid peroxidation in the survival seedlings,the increased activity of CAT and POD could protect them against the damage.
出处
《核农学报》
CAS
CSCD
北大核心
2011年第1期67-70,共4页
Journal of Nuclear Agricultural Sciences
基金
中国农业科学院与北京市大兴区合作项目
关键词
地被菊
种子
辐射诱变
发芽
抗氧化酶
Chrysanthemum morifolium
seeds
irradiation
germination
antioxidant enzymes
