摘要
为了完成生物检测实验中待检测微球的单列通过,基于流式细胞仪的技术原理,设计了一种微流体聚焦芯片,并利用Intellisuite软件对聚焦进行仿真和设计。用微浇铸方法制作基于聚二甲基硅氧烷的微流控芯片,并采用插针式封接,提高了芯片成品率。通过实验制作芯片,并将它应用于测试,成功地实现了直径为10μm的悬浮待检测微球呈单列顺序排列。
In order to make the suspended microspheres to be detected separately and monostichously,based on the technical principles of flow cytometry,a kind of microfluidic focusing chip was designed.The focusing chip was simulated and designed by using software Intellisuite.This PDMS-based microfluidic chip was produced by casting method,adopting a sealing method as contact pins,which greatly improved the yield of chips.Through the experiment,the microfludic focusing chip is fabricated,and is applied to the testing realizing the suspended 10 μm diameter microspheres ordered separate and monostichous.
出处
《吉林大学学报（信息科学版）》
CAS
2011年第1期31-35,共5页
Journal of Jilin University（Information Science Edition）
基金
国家自然科学基金资助项目(07JC2DJC10400)
关键词
聚焦
微流控芯片
生物检测
focusing
microfluidic chip
biological detection
