摘要
目的:通过检测ER、PR、PCNA在子宫腺肌病(Am)腺上皮细胞中的表达,探讨ER、PR在Am细胞增殖活动中的调控作用。方法:采用免疫组化SP法,检测36例Am组患者异位内膜、在位内膜及30例对照组正常内膜中ER、PR、PCNA的表达情况及相关性。结果:异位内膜组ER、PR表达均低于在位内膜(P<0.05)、高于正常内膜(P<0.05);PCNA表达高于在位和正常内膜(P<0.05);正常和在位内膜组ER和PR表达呈中度正相关(P<0.05),ER和PCNA表达也呈正相关(P<0.05)。1级和2级异位内膜组ER、PR表达和PCNA水平均无明显相关性(P>0.05)。在位内膜ER、PR、PCNA的表达增殖期高于分泌期(P<0.05),异位内膜三者表达无周期性(P>0.05)。结论:Am异位内膜组ER、PR与PCNA水平无明显相关关系且缺乏周期性变化,提示异位子宫内膜细胞增殖活动在一定程度上不受卵巢激素的调节,可能异位内膜与在位内膜存在不同的生长环境和增殖分化机制。
Objective：To explore the regulatory effects of estrogen receptor（ER） and progestogen receptor（PR） in cell proliferation of adenomyosis by detecting the expressions of ER,PR and PCNA in epithelial cells of patients with adenomyosis.Methods：Immunohistochemical SP method was used to detect the expression levels of ER,PR and PCNA in eutopic endometrium and ectopic endometrium of 36 patients with adenomyosis and normal endometrium of 30 patients without adenomyosis,and their correlation was analyzed.Results：The expression levels of ER and PR in ectopic endometrium were significantly lower than those in eutopic endometrium（P〈0.05）,and the expression levels of ER and PR in ectopic endometrium were significantly higher than those in normal endometrium（P〈0.05）;the expression level PCNA in ectopic endometrium was significantly higher than those in eutopic endometrium and normal endometrium（P〈0.05）;there was a moderate positive correlation between ER expression and PR expression in normal endometrium and eutopic endometrium（P〈0.05）,and there was a positive correlation between ER expression and PCNA expression（P〈0.05）;there was no correlation between ER expression,PR expression and PCNA expression in ectopic endometrium of grade 1 and grade 2（P〉0.05）;in eutopic endometrium,the expression levels of ER,PR and PCNA at proliferative phase were significantly higher than those at secretory phase（P〈0.05）,the expression levels of ER,PR and PCNA in ectopic endometrium had no periodicity（P〉0.05）.Conclusion：In ectopic endometrium of patients with adenomyosis,the expression levels of ER,PR and PCNA have no correlation and periodicity,which indicates that the cell proliferation of ectopic endometrium can’t be affected by ovarian hormones to some extent,ectopic endometrium and eutopic endometrium may have different growth environment and proliferation-differentiation mechanism
出处
《中国妇幼保健》
CAS
北大核心
2011年第6期903-906,共4页
Maternal and Child Health Care of China
基金
首都医科大学基础-临床科研合作基金〔09JL58〕
