摘要
目的：对西城区两家哨点医院采集的流感样病例咽拭子标本进行监测,以掌握2009年-2010年度西城区流感流行情况。方法：提取咽拭子标本中的病毒核酸,采用荧光定量PCR方法检测甲型H1N1三个主要基因infA、SWinfA、SWH1以及质控RNP基因,然后采用三对基因FluB、FluH1、Flu H3进行季节性流感分型。结果：在监测2117件流感样病例咽拭子标本的核酸检测中,共检出263件甲型H1N1,以10月、11月为高峰;季节性流感的检测中,乙型检出393件,在12月出现上升趋势,1月、2月为高峰;A（H3）检出76件,以8月、9月为流行高峰;A（H1）只检出2件;A未分型15件。结论：西城区2009-2010年度流感流行高峰为2009年10月~2010年3月,以甲型H1N1和B型流感病毒为主,而且在不同时间里出现了不同的优势型别。
Objective：Detection on throat swab specimens from patients suspected influenza of two hospital in Xicheng district in order to analyze the epidemic characteristics of Influenza in 2009-2010.Methods： We have detected virus nucleic acid isolated from specimens on specific genes infA,SWinfA,SWH1 and cotrol gene RNP by real-time RT-PCR,then identifiied the subtype on FluB,FluH1,Flu H3genes.Results： In total 2117 specimens,there were 263 positive samples with influenza A（H1N1）virus subtype which the higest quantity emergered in October and November;in addition to the existence of the seasonal influenza subtypes,the positive of B subtypes were 393 which increased in December,the higest quantity exist in Janurary and Februrary;the positive of A（H3） subtypes were 78 which the higest quantity emergered in August and September;the positive of A1 subtypes were 2,as well as 15 positive of A subtypes undentified.Conclusion： The epidemical peak of influenza in Xicheng district of Beijing was from October 2009 to March 2010 in total years survilance,the predominant type was Influenza A（H1N1） and type B which emergered in differiential time.
出处
《中国卫生检验杂志》
CAS
2011年第1期167-169,共3页
Chinese Journal of Health Laboratory Technology
