摘要
目的:确定双缩脲法测定胸腹水总蛋白和溴甲酚绿法测定胸腹水白蛋白的检测限,并做其检测范围线性评价。方法:制备空白样品,选择近检测限和病理高值的病人样品。在日立7600全自动生化分析仪上分别用双缩脲法测定总蛋白和溴甲酚绿法测定白蛋白,做检测低限和生物检测限分析,采用EP-6P做线性评价和多项式做线性评价。结果:双缩脲法测定胸腹水总蛋白的检测低限为0.47 g/L,生物检测限为1.33 g/L,2.5 g/L^49.7 g/L范围内线性符合临床要求。溴甲酚绿法测定白蛋白的检测低限为0.44 g/L,生物检测限为0.98 g/L,1.2 g/L^35.2 g/L范围内线性符合临床要求。结论:双缩脲法测定胸腹水总蛋白和溴甲酚绿法测定胸腹水白蛋白能满足临床要求。
Objective：Determine the detection limit of hydrothorax and ascites total protein determining with biuret,as well as hydrothorax and ascites albumin determining with bromocresol green method,and perform linearity evaluation of the range of detection.Methods： Prepare blank sample,select samples that are close to the detection limit and those that have high pathology value.Perform total protein determining with biuret,and albumin determining with bromocresol green method on Hitachi 7600 Automatic Biochemic Analyzer,and analyze the lower limit of detection and biologic limit of detection.Apply EP-6P and polynomial to evaluate linearity.Results： By determining hydrothorax and ascites total protein with biuret,the lower limit of detection is determined to be 0.47 g/L,and the biologic limit of detection is 1.33 g/L;The linearity from 2.5 g/L to 49.7 g/L is accepted by clinic.By determining hydrothorax and ascites albumin with bromocresol green method,the lower limit of detection is determined to be 0.44 g/L,and the biologic limit of detection is 0.98 g/L.The linearity from 1.2 g/L to 35.2 g/L is accepted by clinic.Conclusion： The method of determining hydrothorax and ascites total protein with biuret and the method of determining hydrothorax and ascites albumin with bromocresol green method are acceptable clinically.
出处
《中国卫生检验杂志》
CAS
2011年第1期149-151,共3页
Chinese Journal of Health Laboratory Technology
关键词
胸腹水
总蛋白
白蛋白
检测限
检测范围
Hydrothorax and Ascites
Total Protein
Albumin
Limit of Detection
Range of Detection
