摘要
目的:采用酶消化法培养老龄SD大鼠血管平滑肌细胞,为血管疾病,特别是为动脉粥样硬化研究提供大量的原代平滑肌细胞。方法:无菌取老龄SD大鼠主动脉,0.2%Ⅱ型胶原酶消化分离细胞,采用自然纯化、差速贴壁纯化平滑肌细胞,免疫组化鉴定平滑肌细胞α肌动蛋白。结果:免疫组化染色显示细胞纯度在95%以上。结论:酶消化法分离获取SD大鼠平滑肌细胞方法简单,易掌握,采用本方法可稳定获得大量的平滑肌细胞供实验使用。
Objective：To investigate the method of culture aged Sprague-Dawley（SD） rat vascular smooth cells（VSMCs） through enzyme digestion for supplies larges amounts of primary cells for vascular diseases.Methods：VSMCs were isolated from aged SD rat thoracic aortas by enzyme digestion and cultured with the technique of differential anchoring velocity.The expression of α-SMA was dected by immunocytochemistry.Results：Immunohischemistry revealed that the purity of VSMCs exceeded 95%.Conclusion：VSMCs can be gained easily by enzyme digestion in vitro.This method can supply larges amounts of VSMCs for scientific research and experimentation.
出处
《中国当代医药》
2011年第5期15-16,共2页
China Modern Medicine
关键词
血管平滑肌细胞
酶消化法
免疫组化
Α肌动蛋白
Smooth muscle cells
Enzymatic isolation method
Immunohistochemical staining
α-actin
