摘要
在不含缬氨酸（ Ｌ Ｖａｌ）的 Ｍ Ｅ Ｍ 培养基基础上，以普通 Ｍ Ｅ Ｍ 培养基 Ｌ Ｖａｌ（４６ｍ ｇ／ Ｌ）和亮氨酸（ Ｌ Ｌｅｕ）含量（５２ｍ ｇ／ Ｌ）为准，配制 １２ 种限制 Ｌ Ｖａｌ、增加 Ｌ Ｌｅｕ 的不平衡支链氨基酸培养基，用 Ｍ Ｔ Ｔ法观察其对胃癌细胞株 Ｓ Ｇ Ｃ７９０１ 体外增殖的影响和对该细胞株化疗药物敏感性的影响。结果表明，限制 Ｌ Ｖａｌ至正常的 １／４ 对 Ｓ Ｇ Ｃ７９０１ 细胞即有明显的抑制作用（ Ｐ＜ ０．０１），随着 Ｌ Ｖａｌ浓度的继续降低，抑制作用更加显著。增加 Ｌ Ｌｅｕ 至正常的４ 倍，显著增强了 Ｌ Ｖａｌ缺乏对 Ｓ Ｇ Ｃ７９０１ 细胞的抑制作用。不平衡支链氨基酸与抗癌药物５ Ｆ Ｕ、 Ｍ Ｍ Ｃ 和 Ｍ Ｔ Ｘ 有协同作用。提示在氨基酸疗法中，不仅要调整某种氨基酸的绝对量，而且应调整该氨基酸与其他氨基酸的比例。
L valine and L leucine of MEM culture medium were restricted and increased to serial level respectively and used to culture gastric cancer cell line SGC 7901. The growth and chemotherapeutic sensitivity of SGC 7901 in vitro were measured with MTT method. Significant inhibition of SGC 7901 growth presented when L valine was restricted to less than 1/4 of the normal. At the same restricted level of L valine, the inhibition of SGC 7901 was significantly strengthened by increasing L leucine to 4 times of normal. Improved inhibition effect of chemotherapy was achieved at imbalanced branched chain amino acids. The results suggested that proportion of amino acids is more important than absolute levels in adjuvant anti cancer amino acids therapy.
出处
《山东医科大学学报》
1999年第3期223-226,共4页
Acta Academiae Medicinae Shandong
基金
山东省科委科研基金
关键词
支链氨基酸
肿瘤细胞
胃肿瘤
药物疗法
敏感性
Amino acids, branched chain
Tumor cell, cultured
Stoach neoplasms
In vitrom
