摘要
以小麦幼苗的叶、茎和根组织为材料,采用CTAB法和尿素法分别提取其总RNA。结果表明,CTAB法提取的总RNA经电泳检测,28S rRNA和18S rRNA带型完整;A260/A280的比值接近2,纯度较高;将提取的总RNA反转录合成cDNA,可用于RT-PCR分析。尿素法提取叶和茎组织的总RNA质量较好,但提取根组织的效果较差。说明CTAB法较适合小麦幼苗各组织总RNA的提取。同时,两种方法提取过程中均不使用液氮,可节约试验成本。
The total RNA was isolated from young roots,stems and leaves of wheat seedlings by CTAB and urea method.The results showed that the total RNA isolated by CTAB method displayed clear bands of 28S rRNA and 18S rRNA on 1%（w/v） agarose gels;the value of A260/A280 approached 2;and RT-PCR analysis demonstrated that total RNA had high quality.Total RNA was successfully isolated from leaves and stems through urea method,but not from roots,indicating that CTAB method is superior to urea method.At the same time,the liquid nitrogen was not used in present study,thus save the cost of experiments.
出处
《湖北农业科学》
北大核心
2011年第3期463-465,共3页
Hubei Agricultural Sciences
基金
湖北省自然科学基金项目(2004ABA145)
湖北省教育厅青年基金项目(2003B001)
关键词
小麦
总RNA
提取
wheat
total RNA
isolation
