摘要
为探索适宜新疆早熟棉的SSR-PCR反应体系,采用L9(34)正交实验设计,研究了SSR-PCR反应体系的主要成分对扩增结果的影响。结果表明,棉花SSR-PCR最适反应体系为:在10μL的反应体系中,Taq DNA聚合酶用量为0.1 U、dNTP浓度0.2 mmol/L、引物浓度0.4μmol/L、Mg2+浓度2.5 mmol/L和模板DNA 20 ng;在此基础上,从2300对SSR引物中筛选出多态性高的引物52条,分别构建了新疆已经审定的42个早熟棉品种的DNA指纹图谱。该研究为早熟棉品种鉴定、遗传多样性分析,分子标记辅助育种和种子质量综合评价等奠定了基础。
In this study,SSR-PCR reaction conditions of short-season upland cotton in Xinjiang were optimized.The factors that affect SSR results of upland cotton were studied by L9（34） orthogonal design.The results showed that 0.1U Taq Polymerase,0.2 mmol/L dNTP,0.4 μmol/L primer,2.5 mmol/L Mg2＋ and 20 ng template DNA in 10 μL SSR reaction system might be the best combination.52 pairs of SSR primers were screened out from 2300 pairs.The DNA fingerprint for 42 local cultivars of upland cotton in Xinjiang was successfully constructed by SSR-PCR amplification.This study lays a firm foundation for cultivars identification,genetic diversity evaluation and marker-assisted selection breeding and seed quality assessment of short-season upland cotton cultivars in Xinjiang.
出处
《石河子大学学报（自然科学版）》
CAS
2011年第1期1-5,共5页
Journal of Shihezi University(Natural Science)
基金
国家科技支撑计划项目(2007BAD44B07)
关键词
陆地棉
早熟
SSR
体系优化
upland cotton
earliness
SSR
system optimization
