摘要
从完整的小麦种子和它的离体胚成功地诱导出了愈伤组织。愈伤组织诱导和继代培养基中2,4-D、VB_1和肌醇的浓度影响植株再生频率。在分化培养基中加入500mg/L 水解酪蛋白促植再生。KT 对于愈伤组织的生长和分化有抑制作用。不同品种愈伤组织的植株再生能力相差很大,京红8号的植株再生频率较高,为76%。
Callus were induced from epiblast of isolated mature embryos and intact seeds.The concentration of 2,4-D,thiamine and myoinositol in media of callus induction and subculture were factors affecting plant regeneration.Callus pro- liferation and plant regeneration were inhibited by using kinetin.Varieties diffe- red markedly in their callus proliferation and plant regeneration.Plants were regenerated on MS medium supplemcnted with 500mg/lcasein hydrolysate with regeneraiton frequencies of 76% in‘Jing-Hong No.8’variety.
