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RNAi沉默TRF2基因对喉鳞状细胞癌Hep-2细胞生长增殖的影响

Experiment studies on growth and proliferation of Hep-2 cells by silence of TRF2 gene
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摘要 目的:探讨采用RNA干扰技术沉默端粒重复序列结合因子2(TRF2)基因对喉鳞状细胞癌Hep-2细胞生长增殖的作用情况。方法:构建特异性表达TRF2mRNA且含有荧光素基因的shRNA真核表达质粒pshRNA,并将其转染Hep-2细胞,采取PCR琼脂糖凝胶电泳检验靶基因TRF2表达情况,流式细胞仪检测细胞凋亡,cell counting kit-8(CCK-8)法观察细胞增殖活性。结果:Hep-2细胞被转染后,TRF2基因表达明显受抑,细胞凋亡发生,增殖活性明显降低。结论:RNA沉默TRF2可明显抑制人喉鳞状癌细胞的增殖,促进细胞发生凋亡。 Objective：To investigate the effect of RNA interference silencing telomere repeat factor 2 by observing Hep-2 cells＇ proliferation and apoptosis in larynx carcinoma cell line.Method：A recombinant plasmid containing a single shRNA（shTRF2） was constructed.The expression of TRF2 gene was detected by PCR and cell proliferation was examined using CCK-8.Hep-2 cells＇ apoptosis was detected by flow cytometer （FCM）.Result：After treatment with shTRF2,the expression of TRF2 was distinctively depressed,and Hep-2 cells proliferation was obviously inhibited.Compared with control and negative group,cells with treatment of RNAi exhibited significantly more apoptosis.Conclusion：Using RNA interference technique to silence TRF2 gene is effective on inhibiting cancer cells＇ proliferation and help to induce cancer cells＇ apoptosis.
作者 焦路燕 徐勇 陶泽璋
机构地区 湖北省人民医院耳鼻咽喉头颈外科
出处 《临床耳鼻咽喉头颈外科杂志》 CAS CSCD 北大核心 2010年第24期1131-1135,共5页 Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金 国家自然科学基金面上项目(No:30872851)
关键词 喉肿瘤 RNA干扰 端粒重复序列结合因子2 细胞增殖 凋亡 laryngeal neoplasms RNA interference TRF2 cells proliferation apoptosis
分类号 R739.65 [医药卫生—肿瘤]
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参考文献15

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临床耳鼻咽喉头颈外科杂志
2010年 第24期

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