摘要
目的探讨局部应用神经生长因子（NGF）和胰岛素对1型糖尿病大鼠烫伤创面中缺氧诱导因子-1α（HIF—1α）和血管内皮细胞生长因子（VEGF）表达的影响及创面愈合机制。方法雄性wistar大鼠腹腔注射STZ建立糖尿病模型60只，1月后在大鼠背部造成深Ⅱ度烫伤创面，将大鼠随机分为无干预组（B）、胰岛素治疗组（C）、NGF治疗组（D）、NGF联合胰岛素治疗组（E），并设立正常烫伤对照组（A），每组15只。观察伤后3、7、11、15、21d各组创面愈合情况，检测创面组织HIF-1α和VEGF的表达。结果E组创面愈合率第7天为[（25．35±2．32）％，P〈0．05]，与其他各组相比较显著增加；C、D组创面愈合率较B组显著增加分别为[（16．68±1．95）％，（18．29±1．70）％，P〈0．05]；D组自第15天起较A组显著增加[（54．84±3．60）％，P〈0．05]；自第7天起E组HIF-1α和VEGF的表达显著低于其他各组（P〈0．05）。结论局部应用NGF联合胰岛素可加速创面愈合，其机制可能通过促进创面微血管再生，纠正局部缺氧。
Objective To investigate the effects of local application of NGF combined with Insulin on wound healing with II degree deep scald and the expression of HIF-1α and VEGF in diabetic rats. Meth- ods 60 male Wistar rats were used in the study by intraperitoneal injection of streptozotocin （STZ）. Deep partial thickness scalding was engendered on the back of the rats after one month. Then these rats were ran- dom divided into group B （ scalding of the rats with diabetes, n = 15 ）, C （ insulin control, n = 15 ）, D （NGF control, n = 15 ） and E （NGF and insulin supplementation, n = 15 ）. And group A （nomal control, n = 15 ） was created by normal rats of partial thickness scalding. The wound area, wound healing rate, and grey of HIF-1 α and VEGF immunohistochemistry staining were determined on the 3rd, 7th, 15th, and 21st day post scalding days （PSDs）. Results Compared to those in groups A, B, C and D, the wound healing rate in group E increased significantly since the 7th PSD [ （ 25.33 ± 2. 32 ） %, P 〈 0. 05 ]. The wound healing rate in group C and D increased significantly compared with group B [ （ 16. 68 ± 1.95）%, （ 18. 29 ± 1.70）%, P 〈 0. 05 ]. Compared to group A, the group D increased significantly since the 15th PSD [ （ 54. 84 ± 3.60） %, P 〈 0. 05 ]. The expression of HIF-1 α and VEGF in group E began to increase slower than other groups after the 7th day （ P 〈 0. 05 ）. Conclusion Local application of NGF combined with Insulin could be beneficial to the regeneration of capillary vessel in the burn wounds of the rats with diabetes, as well as to accelerate wound healing by redress anoxia and decrease the expression of HIF-1α and VEGF.
出处
《中国医师杂志》
CAS
2011年第1期33-37,共5页
Journal of Chinese Physician
