摘要
目的:研究枇杷幼叶灭菌条件及愈伤组织的诱导、胚状体的分化及植株再生。方法:用饱和漂白粉上清液和0.1%升汞对枇杷叶进行不同时间的灭菌处理;将幼叶切成小块,接种在附加6-BA、2,4-D、NAA的培养基上,进行愈伤组织的诱导和植株再生。结果:幼叶的灭菌处理以在饱和漂白粉上清液中浸泡15 min、在0.1%升汞中浸泡4 min为宜;诱导愈伤组织的最佳配方为6-BA(0.5 mg/L)+2,4-D(0.5 mg/L)+NAA(1.5 mg/L);从愈伤组织诱导胚状体时,添加含不同浓度生长激素的培养基,最佳培养基配方为MS+6-BA(0.5 mg/L)+2,4-D(0.5 mg/L)+NAA(1.5 mg/L),形成了外观质地疏松、颗粒状的黄绿色愈伤组织细胞圆形且形状规则的性愈伤组织;从胚状体中诱导植株再生的最佳培养基配方为MS+6-BA(1 mg/L)+NAA(0.5 mg/L),生根的最佳培养基配方为MS+NAA(0.3 mg/L),移栽成活的最佳基质配比是珍珠岩:泥炭:沙为1:2:1。结论:从幼叶诱导愈伤组织与植株再生,为枇杷苗的规模化生产提供了一条新的途径。
Objective：To study the sterile conditions of leaves,induction of callus,induction of embryogenesis and plant regeneration of loquat.Methods：Loquat leaves were set up different sterilization time by diping in the saturation chloride of lime and 0.1% HgCl2,and were cut into small pieces and inoculated in the medium which added 6-BA,2,4-D and NAA.The leaves callus and plant regeneration were inducted.Results：Saturation chloride of lime dip in 25 min,0.1% HgCl2 dip in 4 min were better for younger leaf.Callus induced from leaves in MS＋6-BA（0.5 mg/L）＋2,4-D（0.5 mg/L）＋NAA（2.0 mg/L） medium.The grain-like,light yellow and loose callus were embryonic callus.During the inducing of embryoid,the culture mediums with different grouth regulator concentration were used.Finally,the culture medium with the highest of reduction frequency was MS＋6-BA（0.5 mg/L）＋2,4-D（0.5 mg/L）＋NAA（1.5 mg/L）.The better culture medium for plant regeneration from embryoid of loquat was MS＋6BA（1 mg/L）＋NAA（0.5 mg/L）.The root medium was MS＋NAA（0.3 mg/L）.The better matrix ratio of transplant was perlite,pert and sand as 1∶2∶1.Conclusion：Induction callus from loquat leaves and plant regeneration are the new way for large-scale production
出处
《生物技术通讯》
CAS
2011年第1期53-56,共4页
Letters in Biotechnology
基金
浙江省自然科学基金(Y307577
Y3090665)
农业部农业公益性行业科研专项(200803034)
关键词
枇杷叶
愈伤组织
胚状体
植株再生
loquat leaves
tissue culture
embryoid
plant regeneration
