摘要
目的:本研究旨在建立SD大鼠胰岛素抵抗模型,观察高脂饲料喂养的SD大鼠肝脏中氧化应激以及胰岛素抵抗的发生,分析胰岛素抵抗状态下活性氧(ROS)的变化,初步探讨ROS的主要来源。方法:以高脂饲料喂养6只4周龄雄性SD大鼠12周,建立大鼠胰岛素抵抗模型。用优越血糖仪以电子感应法测定血糖,放射免疫法检测血清胰岛素水平。二氢乙啶(DHE)染色观察肝脏组织中的ROS水平。Western blotting检测NADPH氧化酶3(NOX3)的表达。结果:以高脂饲料喂养12周后,大鼠空腹血糖水平略有上升,但与对照组的大鼠相比无显著差异,而胰岛素敏感指数降低。蒽酮法的检测结果显示高脂饲料喂养大鼠肝组织糖原含量显著降低,高脂饮食大鼠肝组织中NOX3的表达显著增加,DHE染色显示肝组织ROS水平显著增加,提示ROS在肝胰岛素抵抗发生中起重要作用。结论:高脂饲料喂养SD大鼠胰岛素敏感指数降低,肝组织中NOX3表达和ROS水平显著增加,糖原含量显著降低。
AIM： To observed the relationship between oxidative stress and development of insulin resistance in hepatic tissues of Sprague dawley （SD） rats by analyzing reactive oxygen species （ROS） level and NADPH oxidase 3 （ NOX3 ） expression in livers. METHODS ： Four - week - old male SD rats were fed with high - fat diet containing 20% fat and 20% sucrose for 12 weeks to induce insulin resistance. Plasma insulin level was detected by radioimmunoassay. The content of liver intracellular glycogen was measured using a glycogen assay kit. ROS generation in the liver tissues was assessed by dihydroethidium （DHE） fluorescence. The expression of NOX3 was determined by Western blotting. RESULTS： After 12 weeks of high -fat diet feeding, the content of blood glucose was increased but still maintained in normal level in the rats. However, the index of insulin sensitivity obviously decreased. Hepatic glycogen content in the rats fed with high - fat diet was significantly decreased, indicating that insulin resistance developed. Enhanced ROS production in hepatic tissues of the rats fed with high - fat diet was observed. Importantly, the expression of NOX3 in the liver was up - regulated in response to high - fat diet in vivo. CONCLUSION ： High - fat diet feeding decreases insulin sensitivity, enhances ROS level and NOX3 expression, and reduces glycogen content in the livers.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2011年第2期310-314,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30901577)
湖南省衡阳市科技局资助项目(No.2009KJ14)
