摘要
目的对大鼠海绵体神经(CN)进行解剖并对其造成钳夹、切断损伤,以建立前列腺癌根治术后勃起功能障碍(ED)的动物模型。方法将30只雄性Sprague-Dawley大鼠随机分为CN钳夹组、CN切断组、假手术组,术后4周通过阿朴吗啡(APO)试验及海绵体内压(ICP)/平均动脉压(MAP)测定、阴茎神经型一氧化氮合酶(nNOS)免疫组化检测、荧光金逆行示踪技术,评估建立模型的效果。结果 CN钳夹组、CN切断组大鼠APO试验及电刺激星状神经节(MPG)均无明显勃起反应及ICP/MAP变化,假手术组可见明确勃起反应,并伴有电刺激MPG后ICP/MAP的升高(P<0.05)。阴茎nNOS免疫组化:CN钳夹组、CN切断组大鼠阴茎nNOS阳性神经纤维数明显少于假手术组(P<0.05),而CN钳夹组与CN切断组差异无统计学意义(P>0.05)。荧光金逆行示踪检测:CN钳夹组、CN切断组与假手术组差异均有统计学意义(P<0.05),CN钳夹组与CN切断组差异亦有统计学意义(P<0.05),显示两种损伤方法均能成功造成明确的CN损伤并建立前列腺癌根治术后ED模型,CN切断组大鼠MPG内荧光金阳性细胞数较CN钳夹组少,显示CN切断组较CN钳夹组CN损伤程度更重。结论大鼠CN结构与人类非常相似,CN钳夹、切断损伤均为建立前列腺癌根治术后ED模型的可靠方法。
Objective To establish a rat model mimicking erectile dysfunction following radical prostatectomy by crush injury or rection of the cavernous nerve（CN）.Methods Thirty rats were randomized into CN crush group,CN resection group and sham-operated group.Four weeks after surgery,the rat models were evaluated by apomorphine test and ICP/MAP measurement.Fluorogold（FG） retrograde tracer was used to assess the CN injury.The penile tissues were then harvested for immunohistochemical detection of the nNOS-positive fibers to evaluate the CN injury.Results The rats in CN crush group and CN resection group exhibited erectile dysfunction in apomorphine test or in response to electrical stimulation of the ganglion stellatum（MPG）.In the sham-operated group,the rats showed normal erectile function with increased ICP/MAP following electrical stimulation（P0.05）.Immunohistochemistry revealed reduced nNOS-positive fibers in both CN crush group and CN resection group as compared with those in the sham-operated group（P0.05）,showing no significant difference between the former two groups（P0.05）.The FG-positive MPG cells in CN crush group and CN resection group were significantly less than that in the sham-operated group（P0.05）,and the positive cells were even less in CN resection group（P0.05）.Conclusion The rat CN is structurally similar to human CN,and crush injury and resection of the CN are both reliable methods for establishing rat models of erectile dysfunction following radical prostatectomy.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2011年第2期230-233,共4页
Journal of Southern Medical University
基金
国家自然科学基金(30871571)
广东省科技计划项目(2008B030301059)~~
