摘要
在花生新鲜针叶和干叶ＤＮＡ提取过程中，应用ｐＨ值较低，无机盐浓度较高的提取缓冲液可沉淀蛋白质，其中加入２％的β－巯基乙醇可有效地防止次生代谢物质使ＤＮＡ变色。提取出的ＤＮＡ样品产率在９２．６～２１６．３１ｎｇ／ｍｇ．ｆｗ，ＤＮＡ质量和纯度较高，２６０ｎｍ／２８０ｎｍ光密度比值在１．８～１．９之间。所得ＤＮＡ可直接用于限制性内切酶酶切，并可用于随机引物ＰＣＲ扩增。该方法为花生分子生物学研究提供基础。
A low pH extraction medium with high salts, which avoids ionization and oxidation of phenolic compounds during tissue grinding and precipitation of large amounts of materials, were successfully used to extract total DNA from the leaf of peanut (Arachis hypogaea).The DNA yields, quality and purity were characterized. These extracted DNA could be used directly for RAPD analysis, which are useful as molecular genetic markers, and digested with restriction enzymes. A fast ,inexpensive and reliable procedure has been developed for detecting the genetic diversity ofArachis hypogaea .
出处
《花生科技》
北大核心
1999年第3期1-9,共9页
基金
福建省教委科学研究基金
关键词
花生
DNA提取
内切酶酶切
RAPD
Arachis hypogaea
Isolation of total DNA
Digestion with restriction enzyme
