摘要
目的:研究撤除表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)和血管内皮细胞生长因子(VEGF)对人脐带间充质干细胞(hUCMSCs)的增殖和胞外基质(ECM)表达的影响。方法:将第5代hUCMSCs分3组:对照组(保持上述生长因子浓度不变),半量撤除组(使生长因子浓度减半),完全撤除组(不添加生长因子)。培养30h后瑞特-姬姆萨法染色观察细胞形态,MTT法细胞计数。培养15h后,定量PCR检测BCL-2、BAX及胞外基质基因的表达情况。结果:撤除生长因子后细胞增殖减慢,形态变大,核质比减小,BCL-2/BAX比率增高,胞外基质表达多数下调。半量撤除组细胞的形态、增殖能力、胞外基质表达的改变相对较小,BCL-2/BAX比率增高更明显。结论:撤除生长因子会影响细胞增殖和胞外基质的表达,保留半量生长因子可以改善这种情况。
Objective：To investigate the influence of growth factors withdrawal on the proliferation and extra-cellular matrix gene expression of human umbilical cord mesenchymal stem cells （hUCMSCs）.Method：According to the concentration of growth factors in the medium,the 5th generation hUCMSCs were divided into 3 groups （control group,half amount withdrawal group and complete withdrawal group）. In control group,the concentration of growth factors was the same as that in normal culture medium. The cell morphology was observed by Wright-Giemsa Staining. The proliferation was detected by MTT method and the expressions of BCL-2,Bax and extra-cellular matrix gene were detected with quantitative PCR.Result：After the withdrawal of growth factors,the proliferation of hUCMSCs slowed down and the size of the cells became larger. The BCL-2/BAX ratio increased and the expression of most extra-cellular matrix gene decreased. In half amount withdrawal group,the proliferation,morphology and the expression of most extra-cellular matrix gene just changed a little,while the BCL-2/BAX ratio was higher than that in the complete withdrawal group.Conclusion：Growth factors withdrawal might affect the proliferation and extra-cellular matrix gene expression of hUCMSCs. Half amount of growth factors might maintain the activities of these cells.
出处
《临床血液学杂志》
CAS
2011年第1期42-45,共4页
Journal of Clinical Hematology
基金
山东省自然科学基金资助项目(No:Y2007C109)
山东省科技攻关计划(No:2009GG10002027)
国家自然科学基金项目(No:30700163)
关键词
间充质干细胞
生长因子
增殖
细胞外基质
mesenchymal stem cells
growth factors
proliferation
extra-cellular matrix
