摘要
从4株清酒酵母中筛选获得S-腺苷甲硫氨酸(SAM)积累量较高的WQ3,对该菌株发酵生产SAM的培养条件和分离纯化进行了研究。通过正交试验,得到的优化培养基组成为:蔗糖8%、胰蛋白胨1.2%、L-甲硫氨酸1%、酵母提取物0.75%、MgSO4.7H2O 0.03%、KH2PO40.4%,优化后摇瓶发酵SAM产量可达到2.67 g/L,比优化前提高了19.7%。通过高氯酸破壁、大孔阴离子交换树脂脱色以及大孔阳离子交换树脂分离小分子化合物,SAM分离纯化的总回收率为69.2%。
Saccharomyces sake WQ3 with higher ability of accumulation of SAM was screened out in four strains of sake yeasts tested.The culture conditions of fermentation for this strain to produce SAM and isolation of SAM were studied.Through the orthogonal experiment,the optimization concentrations of medium compositions were determined as follows： sucrose 8%,tryptone 1.2%,L-methionine 1%,yeast extract 0.75%,MgSO4·7H2O 0.03% and KH2PO4 0.4%.Under the optimum medium,the yield of SAM can reach 2.67g/L in erlenmeyer flask which is 19.7% higher than before optimization.Using perchloric acid for breaking yeast cells,macroporous anion exchange resin for decolorization of the extract and macroporous cation exchange resin for separation of SAM from other low molecular weight compounds,the total separation recovery of SAM was 69.2%.
出处
《药物生物技术》
CAS
CSCD
2011年第1期26-31,共6页
Pharmaceutical Biotechnology
基金
江苏省高校"青蓝工程"中青年学术带头人培养对象资助项目
关键词
清酒酵母
S-腺苷甲硫氨酸
发酵
Saccharomyces sake
S-adenosyl-L-methionine
Fermentation
