摘要
外源生长素对白首乌脱分化是必要的，效果最好的是２ ，４ － Ｄ，浓度范围０ ．１ ～０ ．３ｍｇ／ Ｌ；白首乌脱分化的起动和细胞旺盛分裂伴随着内源 Ｉ Ａ Ａ 含量的迅速提高， Ｔｒｐ 含量的变化与 Ｉ Ａ Ａ 相似，但其峰值比 Ｉ Ａ Ａ 提前，这为旺盛分裂期 Ｉ Ａ Ａ 的大量形成奠定基础； Ｄ Ｎ Ａ 和 Ｒ Ｎ Ａ 含量的峰值也出现在旺盛分裂期，这与此时期 Ｉ Ａ Ａ 的大量及时形成和旺盛的代谢活动有关，从形成期直至衰老，内源 Ｉ Ａ Ａ、 Ｔｒｐ 和 Ｄ Ｎ Ａ、 Ｒ Ｎ Ａ 含量下降并保持较低水平。
Auxins were essential for dedifferentiation of Cynanchum bungi Decne. 2,4-D was the most efficacious auxin for dedifferentiation. The suitable concentration range of 2,4-D was 0.3～1.0mg/L. IAA, NOA were the least efficacious auxin for dedifferentiation. The content of endogenous IAA was in raising rapidly during the starting phase and intense division phase. The variation of endogenous Trp was similar to IAA, however it's peak value was earlier than that of IAA. This is favourable for synthetic in time of IAA in intense division phase. The peak values of DNA and RNA were also formed in the phase of intense division This related greatly to the synthetic of IAA and vigorous activity of metabolism in this phase. The contents of endogenous IAA, Trp, DNA and RNA were kept at low level from formed phase to senescence phase.
出处
《山东农业大学学报（自然科学版）》
CSCD
1999年第3期226-230,共5页
Journal of Shandong Agricultural University：Natural Science Edition
关键词
白首乌
脱分化
IAA
核酸
Cynanchum bungi Decne
dedifferentiation
IAA
Trp
nucleic acid
