摘要
目的:观察退热解毒灵水煎剂抑制2009年新型甲型H1N1流感病毒的效果。方法:采用MTT法及细胞病变抑制实验,以利巴韦林为阳性对照,检测退热解毒灵水煎剂对H1N1流感病毒的抑制作用。结果:退热解毒灵水煎剂对体外试验中H1N1流感病毒有一定的抑制作用,其半数中毒质量浓度为8 954.3mg.L-1,抑制H1N1流感病毒的半数有效质量浓度为520.6mg.L-1,治疗指数为17.2。结论:退热解毒灵水煎剂在体外实验中,对H1N1流感病毒有抑制作用。
OBJECTIVE To explore the antiviral effect of decoction of tuirejieduling on pandemic 2009 H1 N1 influenza virus. METHODS The anti-H1N1 influenza virus effect of decoction of Tuirejieduling in MDCK cell culture was investigated by the cytopathic effect （CPE） inhibition assay and MTT assay, with ribavirin as the positive control drug. RESULTS Decoction of Tuirejieduling had inhibitory effect on H1 N1 influenza virus in a certain extent in vitro. The median toxic concentration （TC50） was 8 954. 3 mg·L^-1, the median effective concentration （EC50） was 520. 6 mg·L^-1 and the therapeutic index （TI） was 17.2. CONCLUSION Decoction of Tuirejieduling has inhibitory effect on H1N1 influenza virus in vitro.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2011年第4期277-279,共3页
Chinese Journal of Hospital Pharmacy
基金
武汉市科技计划项目(编号:2009430012)
