摘要
通过在不同pH条件下培养At.f菌,并监测其生长情况,以确定At.f菌生长的最适pH,再向所确定的最适pH的9K培养基中添加不同氨基酸,研究不同氨基酸对At.f菌生长情况的影响。结果表明:pH为2.0的培养基中菌种的生长情况最好;另外丙氨酸、缬氨酸、亮氨酸、异亮氨酸、甲硫氨酸、苯丙氨酸、色氨酸、脯氨酸、酪氨酸、甘氨酸、半胱氨酸对Fe2+的利用率较高,而丝氨酸、天冬酰胺、谷氨酰胺、组氨酸作用结果则相反。从而得出结论:At.f菌生长的最适pH为2.0;非极性氨基酸、成环氨基酸对At.f菌生长有促进作用,丝氨酸、天冬酰胺、谷氨酰胺、组氨酸对At.f菌生长有抑制作用。
Culturing Acidothiobacillus ferrooxidans under the different pH conditions,the growth was monitor to determine the optimum pH,then adding different kinds of amino acids in the 9K medium with the determined optimum pH,the effect of different amino acids on the growth of Acidothiobacillus ferrooxidans were studied.The results showed that：the bacteria was grew best with pH of 2.0;the utilization rate of alanine,valine,leucine,isoleucine,methionine,phenylalanine,tryptophan,proline,tyrosine,glycine and cysteine on Fe2＋were higher,while the action of serine,asparagine,glutamine and histidine were the opposite.It could conclude that：the optimum pH to the growth of Acidothiobacillus ferrooxidans was 2.0;non-polar amino acids and cyclization of amino acids could promote the growth of Acidothiobacillus ferrooxidans,serine,asparagine,glutamine histidine inhibited the growth of Acidothiobacillus ferrooxidans.
出处
《黑龙江农业科学》
2011年第2期16-19,共4页
Heilongjiang Agricultural Sciences
基金
辽宁省教育厅2008年高等学校科研资助计划项目(2008298)
