摘要
本研究利用陆地棉×中棉、陆地棉×海岛棉、独秆突变体×海岛棉、海岛棉×中棉杂交，进行胚珠培养，获得种间杂种植株。结果表明：这４个杂交组合均获得一定频率的胚（包括成熟胚和不成熟胚）及成熟胚萌发的苗；同一组合的杂种胚珠，在不同培养基上获得的胚和成熟胚的频率不同；不同组合的杂交胚珠，在同一培养基上培养的结果有明显差异；进行杂种胚珠培养的适宜时期为授粉后４～６ｄ，２ｄ的胚珠培养易产生愈伤组织，成胚率低，８ｄ后幼胚败育或落桃；适合杂种胚珠早期生理条件的培养基为：ＢＴ基本培养基＋ＮＨ４Ｃｌ１０００ｍｇ／ｌ＋ＩＡＡ１５ｍｇ／Ｌ＋ＫＴ０２ｍｇ／Ｌ＋ＧＡ００６ｍｇ／ｌ；氨态氮能提高成胚率；单一的培养基不能满足杂种胚珠的培养需要。
Ovule
culture was made on Gossypium hirsutumG.arboreum, G.hirsutumG.barbadense, G.vc singal
stem mutantG.barbadense, G.barbadenseG.arboreum. After 8590 days of culture embryos
(including matured and unmatured ones) as well as seedling from matured embryo were
obtained from these 4 crosses with a certain frequency. The results of experiment showed that
the differences were significant between embryo frequency and germination percentage of
embryos with ovule culture on different media and the culture with ovules from different
crosses, and on the same medium the difference was great.\;The ovules from hybrids having
developed for different days after pollination were cultured on BT,BT medium in the darkness.
Embryo development with a high frequency was obtained with ovules from G.hirsutumG.
barbadense and G.hirsutumG. arboreum 46 days after pollination.
出处
《核农学报》
CAS
CSCD
1999年第4期248-252,共5页
Journal of Nuclear Agricultural Sciences
关键词
棉花
远源杂交
不亲和性
胚珠离体培养
杂种植株
Incompatibility of
interspecific hybridization of cotton, ovule culture in vitro, hybrid plants
