摘要
目的调查产ESBLs大肠埃希菌的耐药基因型别分布情况,并对其药敏结果进行分析。方法对临床收集到的227株大肠埃希菌进行常见的6种ESBLs基因PCR扩增,产物利用变性高效液相色谱法进行检测。结果 TEM型的总检出率最高,并且63%以上携带两种以上耐药基因。药敏结果提示,碳青酶烯类抗生素表现100%敏感。结论本研究的调查结果与国内统计资料基本符合,但也表现出一定差异。
Objective To investigate the drug-resistance genotype distribution of Escherichia coli producing ESBLs and analysis its drug sensitivity. Methods PCR were used to amplify 6 ESBLs common gene of E. coli collected from ~linic. PCR amplification products were detected using DHPLC technology. Results The overall detection rate of TEM subtype is the highest,of which more than 63 % carrying 2 resistance genes. Drug sensitivity suggests that Carbapenems showed 100% sensitivity. Conclusion The findings of this study basically consistent with the national statistical data, but also show some difference.
出处
《国际检验医学杂志》
CAS
2011年第1期53-54,86,共3页
International Journal of Laboratory Medicine
关键词
色谱法
高效液相
埃希氏菌属
微生物敏感性实验
chromatograph,high pressure liquid
Escherichia coli
antibiotic sensitivity test
