摘要
目的：研究利钾尿肽及心钠素前体分子内调控作用对心肌Ｎａ＋Ｋ＋ＡＴＰ酶的作用。方法：将大鼠心肌匀浆后，分别加入利钾尿肽、心钠素以及利钾尿肽＋心钠素，用比色法测定Ｎａ＋Ｋ＋ＡＴＰ酶活性。将大鼠心脏悬挂于Ｌａｎｇｅｎｄｏｒｆ灌流装置，分别以利钾尿肽、心钠素、利钾尿肽＋心钠素为灌流液，灌注心脏，用四道生理仪观测左心室内压、左心室收缩最大速率，左心室舒张最大速率，心率及冠脉流量。结果：心钠素虽然对Ｎａ＋Ｋ＋ＡＴＰ酶有抑制作用（抑制率２６．２％），但是，与对照无显著性差异（Ｐ＞０．０５）。利钾尿肽显著抑制酶的活性（抑制率４６．５％，Ｐ＜０．０１）这种抑制作用可被心钠素抵消（抑制率１７．６％，Ｐ＞０．０５）。利钾尿肽可以增加左心室收缩和舒张最大速率以及左室内压，而这种强心作用可因心钠素的加入而消失或减弱。结论：利钾尿肽可以抑制心肌Ｎａ＋Ｋ＋ＡＴＰ酶的活性，产生强心作用，心钠素可以抵消以上作用。
Aim: To study the role of kaliuretic peptide (KP) and
intramolecular regulation of atrial natriuretic factors prohormone in Na + K + ATPase of
myocardium. Methods: After homogenation, the myocardium of rats was divided into 3 groups
and treated with KP, atrial natriuretic peptide (ANP) and KP plus ANP respectively. The activity
of Na + K + ATPase was determined by coloremetry. The hearts of rats were hung on the
Langendorff perfusing apparatus and perfused with KP, ANP, KP + ANP respectively. Left
ventricle press (LVP), maximal systolic and diastolic velocity of left ventricle, heart rate and
coronary flow were observed by 4 leadphy siologic analyzeer. Results: ANP had inhibitory
effect on Na + K + ATPase, inhibition rate (IR) was 26.2%, but not significantly (P>0.05). KP
inhibited the activity of the enzyme (IR was 46.5%, P<0.01) and the effect could be retarded by
ANP(IR was 17.6%, P>0.05). KP could increase the maximal systolic and diastolic velocity of the
left ventricle and LVP and this effect would disappear or attenuate with ANP. Conclusion: KP
can inhibit myocardial Na + K + ATPase activity and produce cardiotonic effect, which can be
offseted by ANP.
出处
《中国应用生理学杂志》
CAS
CSCD
1999年第2期115-118,共4页
Chinese Journal of Applied Physiology
关键词
心钠素前体
心肌
ATP酶
钠
钾
atrial natriuretic factors prohormone
myocardium
Na + K +
ATPase
