摘要
目的探讨LGT蛋白质组在创伤性颅脑损伤(TBI)患者血清中的表达,初步分析其对病情判断的临床意义,并分析多种临床因素对LGT蛋白质组表达的影响。方法采用表面加强激光解析电离飞行时间质谱(SELDI)技术检测105例TBI患者和20名健康对照者血清LGT蛋白质组的表达,比较LGT蛋白质组在不同程度颅脑损伤组和对照组之间的表达差异,并分析手术、濒死等因素对LGT蛋白质组表达的影响。结果在TBI患者血清中存在LGT蛋白质组的表达。在不同损伤程度患者中,重型TBI组和轻中度TBI组LGT蛋白质组丰度明显高于健康对照组(P<0.01);重型TBI组LGT蛋白质组丰度明显高于轻中度TBI组(P<0.01),重型TBI组LGT蛋白质组阳性率明显高于轻中度TBI组(χ2=9.825,P=0.002)。手术后LGT蛋白质组丰度较术前降低,差异有统计学意义(P<0.05)。濒死患者血清蛋白指纹谱明显低平,波峰减少显著,无LGT蛋白质组的表达。结论 TBI患者血清中存在LGT蛋白质组表达谱。LGT蛋白质组丰度的变化反映了颅脑损伤的严重程度,LGT蛋白质组的表达和手术、濒死等因素密切相关,对患者病情判断有重要意义。
Objective To determine the expression of serum lost goodwill target（LGT） proteome as related to prognosis in patients with traumatic brain injury（TBI）,and analyze the effects of a variety of clinical factors on the expression of LGT proteome.Methods The LGT proteome in serum samples from 105 TBI patients and 20 healthy volunteers were detected by SELDI to compare the difference in LGT expression between brain injury group and the control group,and to examine a variety of factors,such as surgery and agonal status,on the expression of LGT proteome were analyzed at the same time.Results The LGT proteome was detectable in the serum of TBI patients.The abundance of LGT proteome in severe TBI group and mild-to-moderate TBI group was significantly higher than that in the controls（P0.01）.The abundance and positive rate of LGT proteome in severe TBI group was significantly higher than that in mild-to-moderate TBI group（χ2=9.825,P=0.002）.Among 17 cases on craniocerebral operations,the abundance of LGT proteome after surgery was lower significantly than that before operation（P0.05）.In agonal patients,the serum protein fingerprints appeared low and flat with significantly fewer peaks,and no expression of proteins was detected.Conclusion The expression of LGT proteome in serum of TBI patients and abundance changes in LGT proteome may reflect the severity of brain injury.The expression of LGT proteome is closely correlated with surgery,agonal status and a few other factors,and thus can be valuable in predicting the prognosis of TBI.
出处
《中国药物与临床》
CAS
2011年第2期125-127,共3页
Chinese Remedies & Clinics
基金
国家高技术研究发展计划(863计划)(2006AA02090406A)
关键词
颅脑损伤
蛋白质组
生物学标记
Craniocerebral trauwa
Proteme
Biological markers
