摘要
目的:探讨Ⅰ、Ⅲ型胶原在正常大鼠胚胎发育不同时期的变化规律及意义。方法:成年健康雌雄大鼠1:1合笼配种,取胎龄10天、12天、14天、16天、18天胚胎皮肤,做石蜡包埋切片,SABC免疫组化染色法检测Ⅰ、Ⅲ型胶原表达。结果:Ⅰ型胶原表达随胎龄的增加表达增加,各胎龄组间差异有统计学意义(P<0.05);Ⅲ型胶原表达随胎龄的增加表达降低,各胎龄组间差异有统计学意义(P<0.05);Ⅰ型胶原与Ⅲ型胶原之间为负相关关系(P<0.05)。结论:Ⅰ、Ⅲ型胶原的表达及其比例与大鼠胚胎皮肤无瘢痕愈合直接相关。
Objective：To explore the change and importance of the expression of collagenⅠandⅢ in skin of mouse fetus.Methods：The paired health grown-up SD rats were sharing a cage.After successful mating,the fetal skin was got at the gestational age of 10 days,12 days,14 days,16 days,18 days,then its paraffin section was used to examine the expression of collagenⅠandⅢby the immunohistochemical method.Results：The expression of collagenⅠwas enhanced with the age of gestation,and there was significant difference between every two groups（P〈0.05）;the expression of collagen Ⅲ was reduced with the age of gestation,and there was significant difference between every two groups（P〈0.05）;there was negative correlation between the expression of collagenⅠand collagen Ⅲ（P〈0.05）.Conclusion：The expression and the rate of the expression of collagenⅠand collagen Ⅲ have correlation to the Scarless Healing of rat fetal skin.
出处
《泸州医学院学报》
2011年第1期10-12,共3页
Journal of Luzhou Medical College
基金
四川省教育厅(2005B013)
四川省科技厅(2008-026)资助项目
