摘要
从平板中直接分离担子菌萌发孢子的单孢分离方法，主要利用自制的毛细管和显徽玻璃针等简单工具操作。该法简便快速，抗污染，获得单孢率高。同时报道一种改进型的担子菌互交不育性测定用培养基MEJA，与国内外目前常用的SR和MEA培养基相比，增加一项判断标准，使互交不育性和互交可育性的反应更加清晰可靠。
A method are described for isolation of single spore. Under the microscope, the germinated single spore of the Aumillaria and other Agaricales can be isolated directly by a handmade pipette and a glass microneedle. the method modified from Kari Korhonen's and operation are better than former. As same times, A improved medium MEJA for intersterility experiments of Armillaria are reposed, by added of fresh malt extract juice to the melt extract powder which were adopted widely, the medium make compatible and incompatible reaction clarify exactly.
出处
《微生物学通报》
CAS
CSCD
北大核心
1999年第3期207-209,共3页
Microbiology China
基金
国家自然科学基金青年基金!39500001
