摘要
研究了在极端嗜盐菌的液氮超低温冻结保藏和真空冷冻干燥保藏中，不同种类的保护剂和保护剂的不同盐浓度对存活性的影响．结果表明：在极端嗜盐菌的保藏中，一定浓度的NaCl是保护剂中不可缺少的组成成分之一，但保护剂的盐浓度没有必要达到或接近生长需要的最适盐浓度（20％—25％NaCl）；在真空冷冻干燥保藏中，用6％的海藻糖代替脱脂牛奶作为保护剂，可以得到较高的细胞存活率。77株嗜盐古细菌保藏12个月后检测，全部存活，其中19株菌保藏24个月，检测结果为全部存活．
Viability of the frozen cultures and freeze--dried cultures of 3 strains by using different concentrate of salt and different protectant was examined. The result clearly show that the protectant should contain swdium chloride for preservation of extreme halobacteria But it is not necessary that the salt concentration of protectant must be the same as it for growth. And 6% behalose as a Protectant for freeze-crying is more better than skimmed milk. Viability of the frozen and freeze-dried cultures of 77 strains after one year and 19 strains after two years was 100%.
出处
《微生物学通报》
CAS
CSCD
北大核心
1999年第3期201-203,共3页
Microbiology China
