摘要
探讨了在富Ｎ条件下白腐菌降解芳香化合物酶的活性变化和对联苯的降解．研究结果表明，ｍ（Ｃ）／ｍ（Ｎ）比值对白腐菌的ＬｉＰ和ＭｎＰ活性有明显的影响；黎芦醇提高了ＬｉＰ和ＭｎＰ的活性及培养基中·ＯＨ基数量。
Activity changes of the enzymes for degrading aromatic compounds and cometabolism of diphenyl and veratryl alcohol were studied by using white rot fungus Phlebia radiata I 5 6 under N rich nutritions. The results showed that m(C )/m(N) concentration ratio obviously affected the activity of LiP. Under high concentration ratio of m(C)/m(N) or m(C)/m(N) a larger amount of LiP was produced, but higher activity of MnP could only be produced by the lower concentration of C or N source. When the conentration of C or N surpassed 0.01 mol/L, the activity of MnP dropped down. Veratryl alcohcol increased the activity of LiP and MnP, as well as the amount of ·OH free radicals under various concentrations of N or C source. Ascorbic acid, a kind of scavenger of ·OH radicals free, produced the opposite effect on the LiP and MnP activity, but the two did not affect the biomass of hypha. Under N rich nutrition conditions the degradation of diphenyl was slow, but under cometabolic conditions of veratryl alcohol and diphenyl, the degradation of diphenyl was enhanced obviously. Morever, no intermediate products during the degradation process were accumulated in the culture media.
出处
《应用与环境生物学报》
CAS
CSCD
1999年第3期320-325,共6页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金
广东省自然科学基金
中科院广州化学所纤维素开放实验室开放基金
中科院广州化学所有机地球化学国家重点实验室资助项目
关键词
白腐菌
富N
共代谢
联苯
降解
white rot fungus Phlebia radiata
veratryl alcohol
N rich condition
cometabolism
