摘要
以２１株分离自四川、云南不同花生产区的慢生型花生根瘤菌和６株参比菌株为材料，进行了１６ＳｒＤＮＡＰＣＲＲＦＬＰ，结果除证实了慢生型花生根瘤菌同慢生型大豆根瘤菌（Ｂｒａｄｙｒｈｉｚｏｂｉｕｍｊａｐｏｎｉｃｕｍ）高度的遗传相似性外，还发现一些菌株同Ｂｒａｄｙｒｈｉｚｏｂｉｕｍｌｉａｏｎｉｎｇｅｎｓｉｓ和Ｂｒａｄｙｒｈｉｚｏｂｉｕｍｅｌｋａｎｉ有较高的遗传相似性，菌株Ｓｐｒ１２的１６ＳｒＲＮＡ部分碱基序列同Ｂｒａｄｙｒｈｉｚｏｂｉｕｍｅｌｋａｎｉ模式菌株ＵＳＤＡ７６的部分序列完全一样，从而证明我国的花生根瘤菌之间具有种水平的多样性．
S rDNA PCR RFLP of 6 referent strains and 21 peanut bradyrhizobial strains isolated from different regions in Sichuan and Yunnan provinces was analyzed and the partial sequence (from 1～800 bp) of 16S rRNA of Spr1 2 was tested. The results showed that high homology in genotype among Bradyrhizobia sp.( Arachis ), B . japonicum , B . elkanii and B.liaoningensis was exited, and the partial sequence (1～800 bp) of 16S rRNA of Spr1 2 was exactly same as that of USDA76 (the type strain of B. elkanii ). Apparently, the biodiversity at the level of species is exited among Chinese peanut bradyrhizobial strains.
出处
《应用与环境生物学报》
CAS
CSCD
1999年第3期315-319,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金
欧盟国际合作项目
