摘要
目的分析Del红细胞膜表面D抗原表位并测定其抗原强度。方法采用16种针对D抗原不同表位的单克隆抗体,通过吸收放散方法检测74例Del个体红细胞膜表面D抗原表位,应用流式细胞术检测其相对抗原强度。结果 74例Del个体D抗原1~9表位检测均为阳性,D阴性、Del及D阳性红细胞相对D抗原阳性率分别为(0.97±0.18)%、(3.06±0.31)%和(99.65±0.71)%,3种表型红细胞D抗原平均荧光强度分别为13.89±2.45,24.18±3.38,223.32±13.05。结论 Del红细胞膜表面D抗原表位表达基本完整,其D抗原强度极低,仅含有微量的抗原分子数目。
Objective To determinate D antigen epitopes and intensity for DEL phenotype.Methods Red blood cell samples from 74 Del individuals were analyzed with 16 anti-D monoclonal antibodies specific for 1~9 epitopes of D antigen through absorption-elution test,and then the relative antigen intensity were detected by flow cytometry.Results A total of 1~9 epitopes of D antigen were tested positive in all 74 Del individuals.The positive rate of relative antigen for D（-）,Del,and D（＋） were（0.97±0.18）%,（3.06%±0.31）%,（99.65±0.71）%,respectively.The Geo Mean fluorescence intensity of D antigen for the red blood cells of the three phenotype were 13.89±2.45,24.18±3.38,223.32±13.05,respectively.Conclusion The red blood cells of Del individuals express grossly complete epitopes and have very low antigen intensity and only contain very small number of D antigen molecules.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2011年第1期23-26,共4页
Chinese Journal of Blood Transfusion
基金
国家自然科学基金(30972815)
