摘要
目的建立RP-HPLC法测定葛根芩连片中盐酸小檗碱含量的方法。方法采用反相高效液相色谱法,用AgilentZORBAX SB-C18柱(4.6×250mm,5μm),以乙腈-0.05mol/L三乙胺溶液(用磷酸调节pH至3.0)(25∶75)为流动相,流速为1.0mL/min,检测波长为347nm,柱温为室温。结果 RP-HPLC法测定方中盐酸小檗碱的含量在0.16-0.80μg范围内线形关系良好,r=0.9993,平均加样回收率为97.0%,RSD=1.3%。结论本法简便,准确,重现性好,可用于葛根芩连片的质量控制。
Objective To establish a RP-HPLC method for the determination of berberine hydrochloride in Gegen Qinlian Tablet.Methods The RP-HPLC conditions with Agilent ZORBAX SB-C18 column（4.6×250mm,5μm）was used,the mobile phase was a solution of acetonitrile-0.05mol/L triethyamine（phosphase was used to adjust its pH to 3.0）（25∶75）,flow rate was 1.0 mL/min,the detective wavelength was 347nm,column temperature was room temperature.Result The determination of Berberine Hydrochloride in Gegen qinlian pian by RP-HPLC was a good linear relationship at the range of 0.16 to 8.0μg,r=0.9993.The average recovery rate was 97.0% with RSD=1.3%.Conclusions This method is simple,accurate and with good reproducibility,and can be used as the quality control standard for Gegen qinlian pian.
出处
《湖北中医学院学报》
2011年第1期30-31,共2页
Journal of Hubei College of Traditional Chinese Medicine
