摘要
【目的】高血脂是公认的心血管疾病的危险因素,本实验拟建立一种昆明小鼠通过注射poloxamer 407而形成高血脂的动物模型。【方法】雄性昆明小鼠随机分为对照组和poloxamer 407组。动物禁食过夜后,一次性腹腔注射poloxamer 407(0.3 g/kg),于注射前及注射后的3、6、24、48及72 h,分别取血测定血中的甘油三酯和胆固醇含量。【结果】poloxamer 407可使昆明小鼠的血甘油三酯和血胆固醇分别升高至基础值的40倍和5倍,且高血脂状态至少能维持72 h以上。【结论】Poloxamer 407能诱导昆明小鼠形成高血脂模型。
【Objective】 Hyperlipidemia is a well-established risk factor for cardiovascular morbidity.We attempted to develop a chemically-induced hyperlipidemic animal model in Kunming mice,using poloxamer 407.【Methods】 Male Kunming mice were randomly divided into 2 groups：control group without any intervention and poloxamer 407 group.After fasted overnight,a bolus of poloxamer 407（0.3 g/kg） was intraperitoneally injected into mice,and blood triglyceride and cholesterol were measured at 0,3,6,24,48 and 72 h after the injection.【Results】 Poloxamer 407 caused the increase of blood triglyceride more than 40-fold and cholesterol 5-fold.And this hyperlipidemic effect of poloxamer 407 lasted up to 72 h after injection.【Conclusions】 Poloxamer 407 induced Kunming mice may be used as a new hyperlipidemic animal model.
出处
《武警医学院学报》
CAS
2011年第2期85-87,96,共4页
Acta Academiae Medicinae CPAPF
基金
武警医学院院级科研基金项目(WBS200905)
