摘要
目的观察清胃理肠胶囊对溃疡性结肠炎大鼠结肠病理变化和T淋巴细胞亚群的影响。方法用二硝基氯苯-乙酸复合法制作溃疡性结肠炎大鼠模型,灌胃给药20d后,肉眼及光镜下观察结肠病理变化,流式细胞仪检测外周血CD4+、CD8+百分率,并计算CD4+/CD8+。结果造模后大鼠结肠肉眼可见溃疡、糜烂,大面积覆盖脓苔;镜下肠黏膜大面积受损,形成融合性溃疡,肠壁炎细胞浸润,组织水肿;CD4+、CD8+百分率降低,CD4+/CD8+升高。清胃理肠胶囊治疗后,结肠病理变化基本消失,与模型组比较CD4+、CD8+升高,CD4+/CD8+降低。结论清胃理肠胶囊能够改善溃疡性结肠炎大鼠结肠的病理变化,并调节其免疫功能。
Objective：To observe the effects of Qingweilichang Capsule on pathological change and T lymphocyte subgroups in rats with experimental ulcerative colitis（UC）.Methods：The UC models were induced in all the rats except the normal group by the combination of dinitro-chlorobenzene and acetic acid.After gastric perfusion for 20 days,the pathological changes were observed by the naked eye and the microscope.Results：After model establishment,the inflammatory reactions were reduced including the tissue ulceration,the cell infiltration and the tissue edema.The CD4＋ percent and the CD8＋ percent were decreased.The CD4＋/CD8＋ was increased.After treatment by the Qingweilichang Capsule,these inflammatory reactions were disappeared.Compared with the model group,the CD4＋ percent and the CD8＋ percent were increased.The CD4＋/CD8＋ was fallen.Conclusions：Qingweilichang Capsule can improve the pathological changes and regulate the immunology functions of rats with UC.
出处
《中国中医急症》
2011年第2期262-263,共2页
Journal of Emergency in Traditional Chinese Medicine
基金
陕西省科技攻关项目[2006K13-G4(1)]
